Published in Cancer Detection and Prevention 1996; 20(5).

Karyotype analysis of pm by Y and ploidy by LSC for differential diagnosis of hydropic chorionic villi

M Ohmori MD PhD, H Miki MD PhD, S Kobayashi MD PhD, E Ohmori MD, M Nakano CT

AIM: Great majority of partial mole (PM) has triploid karyotype, 69XXY and diandric constitution. Histopathological diagnosis is sometimes difficult to distinguish a PM from a complete mole or a hydropic abortus by the small biopsy specimens. However, the distinction is important from a prognostic standpoint. METHODS: We analyzed the nuclear ploidy by laser scanning cytometer (LSC) and detected the 149-bp Y-chromosome-specific gene, Kogan segment (Y) by the PCR method using human Y-chromosome-specific primer from deparaffinized specimens of 16 PM diagnosed by histological criteria. RESULTS: fourteen of the 16 cases were triploid and the remaining two cases were not determined. Y was detected in 12 of the 16 cases. Taken together, the karyotype was 69XXY in 11 cases (69%), 69XXX in 3 cases (19%), and not determined in two cases (12%). CONCLUSIONS: a combination of DNA LSC analysis and Y detection is a sensitive and helpful means for analyzing karyotype in PM even with small specimens from paraffin-embedded materials, and it offers an informative supplement to the histological interpretation of PM.

KEY WORDS: partial mole, karyotype, .

Paper presented at the International Symposium on the Impact of Cancer Biotechnology on Diagnostic and Prognostic Indicators; Nice, France; October 26 - 28, 1996; in the section on Uterus.

http://www.cancerprev.org/Journal/Issues/20/5/304/1145