Published in Cancer Detection and Prevention 1999; 23(4):325-332.

A Receptor Tyrosine Kinase, UFO/Axi, and Other Genes Isolated by a Modified Differential Display PCR are Overexpressed in Metastatic Prostatic Carcinoma Cell Line DU145

A. N. K Jacob, Ph.D.,a John Kalapurakal, M.D.,b William R. Davidson, B.S.,a Geeta Kandpal M.S.,a Naomi Dunson,a Yatindra Prashar, Ph.D.,c and Rajendra P. Kandpal, Ph.D.a

a Fels Institute for Cancer Research and Molecular Biology and b Deparment of Radiation Oncology, Temple University School of Medicine, Philadelphia, Pennsylvania, and c Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut

Address all correspondence and reprint requests to: R. Kandpal, Ph.D., Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad St., Philadelphia, PA 19140.

ABSTRACT: We have used a modified differential display PCR protocol for isolating 3’ restriction fragments of cDNAs specifically expressed or overexpressed in metastatic prostate carcinoma cell line DU145. Several cDNA fragments were identified that matched to milk fat globule protein UFO/Axi, a receptor tyrosine kinase, human homologue of a Xenopus maternal transcript, laminin and laminin receptor, human carcinoma-associated antigen, and some expressed sequence tags. The transcript for milk fat globule protein, a marker protein shown to be overexpressed in breast tumors, was elevated in DU145 cells. The expression of UFO/AxI, a receptor tyrosine kinase, was considerably higher in DU145 cells as compared to normal prostate cells and prostatic carcinoma cell line PC-3. The overexpression of UFO oncogene in DU 145 cells is discussed in the context of prostate cancer metastasis.

KEY WORDS: differential display, metastasis, oncogene, prostate, receptor tyrosine kinase, UFO/AxI.