Published in Cancer Detection and Prevention 2000; 24(Supplement 1).

Prognostic value of DNA ploidy response to chemotherapy in human osteosarcomas

K Kusuzaki MD 1, H Takeshita MD 1, H Murata MD 1, S Hashiguchi MD 1, M Hirata MD 1, T Ashihara MD 2, Y Hirasawa MD 1

1 Dept Orthopaedic Surgery,, 2 Pathology, Kyoto Prefectural University of Medicine, Kyoto, Japan,

AIMS: We conducted to clarify the prognostic value of DNA ploidy response to chemotherapy in human osteosarcomas. METHODS: The DNA ploidy alterations after preoperative chemotherapy in 30 patients with osteosarcoma were analyzed by DNA cytofluorometry. RESULTS: 12 diploid and 9 non-diploid tumors did not change their ploidy pattern after chemotherapy, but 9 non-diploid tumors changed to a diploid pattern with disappearance of the aneuploid cells. These 9 patients with altered ploidy tumors had a better histologic response to chemotherapy and a better prognosis than the patients with non-altered tumors especially diploid tumors (p=0.0138) CONCLUSIONS: Therefore, we conclude that a decrease in aneuploid cells after chemotherapy is closely correlated with a good prognosis and speculate that aneuploid cells may be more chemosensitive than diploid cells in osteosarcomas.

KEY WORDS: fluorescence, smear cells, doxorubicin, cisplatin, tumor necrosis, pulmonary metastasis.

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Paper presented at the International Symposium on Impact of Biotechnology on Cancer Diagnostic & Prognostic Indicators; Geneva, Switzerland; October 28 - 31, 2000; in the section on prognostic markers.