Published in Cancer Detection and Prevention 2000; 24(Supplement 1).

The investigation of oleanolic acid and ursolic acid combined with high dose ionizing radiation on the apoptosis of HL-60 cells

HY Hsu PhD 1, LH Tsai MS 2

1 School of Life Science,, 2 Dept Biochemistry, Tzu-Chi University, Hualien, Taiwan,

AIMS: To evaluate the active components in the extracts of Peh-hue-juwa-chi-Cao and their effects on tumor cells when combined with radiation. METHODS: Anti-tumor components, oleanolic acid (OA)and ursolic acid (UA), were extracted and purified to treat the exponentially growing HL-60 cells with various doses alone or combined with different dose of radiation. We evaluate the cellular damages in HL-60 cells by MTT assay, gel electrophoresis and structural observation. RESULTS: HL-60 cells were decreased significantly at 12 hrs after OA or UA treated with a dose more than 50 ug/ml. Combining with radiation didn't accelerate the death of cells, however, increased the survival of cells. CONCLUSIONS: The cellular damage induced by OA and UA may be disturbed by radiation. The more dose of radiation used, the more inhibitory effect of both OA and UA on HL-60 cells can be found.

KEY WORDS: oleanolic acid, ursolic acid, radiation, apoptosis, HL-60 cells.

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Paper presented at the International Symposium on Impact of Biotechnology on Cancer Diagnostic & Prognostic Indicators; Geneva, Switzerland; October 28 - 31, 2000; in the section on synergistic therapies.