Published in Cancer Detection and Prevention 2001; 25(3):223-234.

Comparative Analysis of Two-Dimensional Protein Patterns in Malignant and Normal Human Breast Tissue

Klaus F. Czerwenka, MD,a Mahmood Manavi, MD,b Josef Hosmann, MD,b Darko Jelincic, MD,b Kerstin I. D. Pischinger, MSc,a Walter B. Battistutti, DVM,a Mark Behnam, BA,a and Ernst Kubista, MDb

aDepartment of Gynecopathology, and bGynocology and Obstetrics, General Hospital, University of Vienna, Vienna, Austria.

Address all correspondence and reprint requests to: Klaus Czerwenka, MD, Department of Gynecopathology, General Hospital, University of Vienna, Vienna, Austria.

ABSTRACT: Malignant and normal human breast tissue were compared by evaluating two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) maps of frozen tissue samples. Image analyzing software was used to scan and process 34 gels. Eight (8/34) of these gels (4 malignant breast tumor samples, 4 normal tissue samples) were selected on the basis of gel and image quality to build a database to identify and measure the expression of a previously inidentified proteome. Growth factor receptor proteins (GFRs), including ERBB2 (HER2) and ERBB3 (HER3), were expressed in the malignant tissue samples. Growth factor receptor proteins were not expressed in the normal tissue. Also, expression of PS2-protein (pS2) was detected in neither malignant nor normal tissue. In benign breast samples a higher intensity of protein expression could be observed for maspin, desmoglein 3 and keratin 8 than in malignant samples. Other proteins expressed in malignant breast tissue include mitogen-activated protein kinase 3 (MK03), heat shock protein 27 kDa (HS27), growth factor receptor-bound protein (GRB2), cathepsin D, G1/S specific cyclin E1 (CGE1), glucose transporter type 5 (GTR5), and a number of as yet unidentified proteins.

KEY WORDS: breast tumor, two-dimensional gel electrophoresis, proteome.