The cAMP responsive element a mediator construct between nerves and enteroendocrine cell development

GE Holle, MD,J Dietl, MD, I Demir,MD

In the present study we elucidate the influence of the enteric innervation on enteroendocrine cells (EECs) by intramural denervation and account for the deviations as a common genetic expression of both systems. In an immunohistochemical and morphometric study D-cells, N-cells, I-cells, L-cells and 5-HT i.r.cells were counted in the rat duodenum, proximal and distal jejunum and iliem before and 21 days after chemical myenteric ablation. Besides an increase in the mucosal crypt depth with increased proliferation(3H-index)and villus hight EECs showed differentiated spatial and numerical changes expressed as percentage(%) of 20000 counted epithelial cells. The genetic expression of the EEC-product and of the transmitter of the submucosal nerves and their receptors at the crypt cells elucidated a common cAMP responsive element (CRE) and their binding protein (CREB) out of the bZIP family of transcription factors. An enteric nervous control of the expression of EECs based on the common genetic construct must be assumed.

KEY WORDS: enteric nervous system (ENS), enteroendocrine cells (EECs), cAMP.

For more information, contact HolleGResearch@aol.com

Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Paris, France; February 9 - 12, 2002; in the section on Gene Therapy, Part 1.

http://www.cancerprev.org/Journal/Issues/26/101/994/4674