The primary study of selenium-methyl-cysteine on differentiation of neural stem cell in vitro
The Department of Pathology of Shantou University Medical College, The Department of Pathology of Shantou
Selenium is very important to the embryo and brain development. Along with the technology of neural stem cell(NSc) isolation and cultivation in vitro is established, the neural development biology is great promoted. In order to assess how selenium to affect NSc in vitro, we apply inorganic sodium-selenite, organic selenium-methyl-cysteine as well as the successfully culture primary and subculture the newborn S-D rat NSc, to observe the function of selenium to the NSc development and differentiation. At the same time, we use the method of immunocytochemisty and slide cultivation to observe the neurosphere morphology, neuritic outgrowth and identify the Nestin, NSE and GFAP et expression. We find that appropriate selenium promotes the neurosphere development and differentiation, the cell grows better, the livability is higher,with typical morphology, more quantity, more branches and good structure of nerve net, Compare with the sodium selenite the selenium-methyl-cysteine has more function. Conclusion: the appropriate selenium especially selenium-methyl-cysteine have very important effect for the NSc development and differentiation.
Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Nice, France; February 7 - 10, 2004; in poster session 793 (Dietary Influences).