(-)-Menthol induced modulation of cell cycle in gastric cancer cells
aSchool of Chinese Medicine, bCenter of General Education, cDepartment of Microbiology; China Medical University, Taichung 400, Taiwan
We have investigated the chemopreventive role of (-)-menthol in human stomach adenocarcinoma cells (SC-M1) by studying the regulation of proliferation and cell cycle arrest. The examination of proliferation was determined by MTT array and Flow cytometry. The examination of cell cycle was determined by flow cytometry. The examination of cyclins and cyclin-dependent kinases were determined by western blotting and flow cytometry. (-)-Menthol inhibited cell proliferation and induced G1/M arrest in SC-M1 cells. SC-M1 cells were treated with various concentrations of (-)-menthol in vitro. The growth of cells and cell cycle was inhibited by (-)-menthol in a time-and dose-dependent manner. Investigation of the levels of cyclins E, D1, D2, D3, and B by PCR, cDNA Microarray and immunoblot analysis showed cyclin B level was unaffected, whereas cyclins D1, D3, and E levels declined with (-) menthol in SC-M1 cells. Investigation of cyclin-dependent kinases, CDK1, and CDK2 show the levels of both kinases decreased markedly in response to (-)-menthol.
Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Nice, France; February 7 - 10, 2004; in poster session 798 (Growth factors & signalling).