Mycobacterium leprae multiplied well in IL-10 intense host
Leprosy Research Center, National Institute of Infectious Diseases. 4-2-1, Higashimurayama-shi, Tokyo, Japan
AIM: We have established a congenic hypertensive nude rat strain,SHR/NCrj-rnu which is highly susceptible to Mycobacterium leprae(M.leprae). In order to examine the reason why SHR/NCrj-rnu have an excellent high susceptibility to M.leprae, cytokine production were detected from peritoneal macrophages. Methods: The culture supernatants of the resident and M.leprae-infected peritoneal macrophages of SHR/NCrj-rnu and F344/NJcl-rnu rats were collected and IL-1α, IL-6, IL-10, IL-12, TNFα and IFNγ production was determined by ELISA. Results: The resident peritoneal macrophages of SHR/NCrj-rnu rats produced IL-1α, IL-6, IL-10 and TNFα, whereas those of F344/NJcl-rnu rats produed only TNFα. Although, M.leprae-infected peritoneal macraophages of SHR/NCrj-rnu rats produced IL-10. However, those of F344/NJcl-rnu rats never produced IL-10 even stimulated with M.leprae.
Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Nice, France; February 7 - 10, 2004; in poster session 892 (Susceptibility genes).