Expression of CD44V6 gene in normal human peripheral blood
Department of Pathology, Institute of Molecular Pathology, Medical School of Southeast University, Nanjingï¼210009, PR, China
Whether CD44v6 gene expression in human peripheral blood could be used as a molecular marker of cancer metastasis is controversial. We investigated D44v6 gene expression in 50 normal human peripheral blood in this study. RNA was purified from the peripheral blood mononuclear cells of healthy donors, subsequently using reverse transcriptase-polymerase chain reaction (RT-PCR) to investigate the expression of CD44v6. CD44v6 mRNA was detected by 58% of healthy volunteers under the proper controls. Our results suggest that the measurement of CD44v6 expression in the peripheral blood by RT-PCR is not suitable for detection of circulating tumor cells.
Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Nice, France; February 7 - 10, 2004; in poster session 893 (Molecular pathology).