Bone marrow derived osteoprogenitor cells can afford osteogenic ability to radiated bone

T Morishita MD^a, Y Touma MD^a, H Ohgushi MD^b, K Honoki MD^a, Y Miyauchi MD^a, S Miura MD^a, A Kido MD^a, K Yoshitani MD^a, Y Takakura MD^a

^aNara Medical University Department Orthopedic Surgery Shijo-cho 840, Kahihara, Nara, Japan, ^b

AIM: We attempted to confirm whether bone marrow derived osteoprogenitor cells can afford osteogenic ability to radiated bones which lost their osteogenic potential by irradiation. METHODS: Fischer 344 male rats were used. Bone marrow cells were collected from seven-week-old Fischer 344 rats and expanded ex vivo. After one week primary culture, the osteogenic progenitor cells were collected by trypsine treatment and then, cell suspension were made for bone and cell composites. Bones were collected from iliac bones and prepared by 60Gy irradiation. Then, radiated bones were soaked in the marrow-cell suspension for thirty minutes, approximately. Thereafter radiated bones with/without osteoprogenitor cells and bones without any treatments were transplanted into subcutaneous spaces. After four and eight weeks samples were collected and prepared for histological findings. RESULTS: We found osteoblasts in some lacunae in radiated bones with cells four weeks after implantation. Newly formed bones surrounded by osteoblast and chondrogenic area were found in radiated bones with cells eight weeks after implantation. However, empty lacunae only were found in bones without cells. CONCLUSION: We confirmed the bone marrow derived cells can afford osteogenic ability to the radiated bones by histological assessment.

Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Nice, France; February 7 - 10, 2004; in oral session 898 (Oncogenesis).